Multi-photon confocal microscopy by using a femtosecond Cr forsterite laser

Author

Tze-Min Liu ; Shi-Wei Chu ; Chi-Kuang Sun ; Ping-Chin Cheng

Author_Institution

Graduate Inst. of Electro-Opt. Eng., Nat. Taiwan Univ., Taipei, Taiwan

fYear

2000

fDate

10-15 Sept. 2000

Abstract

Summary form only given. Confocal laser scanning microscopy provides a significant improvement in axial resolution over conventional epi-fluorescence microscopy by eliminating out of focus fluorescence using a spatial filter in the form of a confocal aperture. In this presentation, we will present our recent studies on two-photon confocal scanning microscopy by using a femtosecond Cr:forsterite laser with output wavelength centered at 1220-1240 nm.

Keywords

biological techniques; chromium; fluorescence; high-speed optical techniques; multiphoton processes; optical focusing; optical microscopy; solid lasers; spatial filters; 1200 to 1240 nm; Mg/sub 2/SiO/sub 4/:Cr; axial resolution; confocal aperture; confocal laser scanning microscopy; epi-fluorescence microscopy; femtosecond Cr forsterite laser; femtosecond Cr:forsterite laser; multi-photon confocal microscopy; out of focus fluorescence; output wavelength; spatial filter; two-photon confocal scanning microscopy; Biomedical optical imaging; Chromium; Focusing; Influenza; Infrared imaging; Luminescence; Microscopy; Optical beam splitting; Power lasers; Spatial resolution;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics Europe, 2000. Conference Digest. 2000 Conference on

Conference_Location

Nice

Print_ISBN

0-7803-6319-1

Type

conf

DOI

10.1109/CLEOE.2000.909843

Filename

909843