Title :
Changes in collagen cross-linking of articular cartilage are revealed by spectral reflectance imaging
Author :
Kinnunen, Jussi ; Kokkonen, Harri T. ; Kovanen, Vuokko ; Hauta-Kasari, Markku ; Vahimaa, Pasi ; Jurvelin, Jukka S.
Author_Institution :
Depts. of Phys. & Math., Univ. of Eastern Finland, Joensuu, Finland
Abstract :
Excessive cross-linking of collagen during aging may contribute to degeneration of articular cartilage. Traditionally, the amount of cross-links is derived by using destructive high-performance liquid chromatography (HPLC). However, a sensitive, nondestructive method could help to evaluate tissue integrity, including cross-links. We increased collagen cross-linking in bovine articular cartilage by controlled threose incubation. During the incubation, optical spectral reflectance images of the samples were captured and related to HPLC results using regression analysis. Significant correlations with reflectance and cross-links were observed. When further developed the optical method may enable nondestructive evaluation of cross-links in vivo through a clinical arthroscope.
Keywords :
biomedical optical imaging; molecular biophysics; proteins; regression analysis; articular cartilage collagen cross-linking; bovine articular cartilage; clinical arthroscope; nondestructive evaluation; optical spectral reflectance image; regression analysis; spectral reflectance imaging; threose incubation; Bovine; Educational institutions; Imaging; Liquids; Optical reflection; Reflectivity; Regression analysis;
Conference_Titel :
Computer-Based Medical Systems (CBMS), 2012 25th International Symposium on
Conference_Location :
Rome
Print_ISBN :
978-1-4673-2049-8
DOI :
10.1109/CBMS.2012.6266357
