DocumentCode :
2950934
Title :
On-chip integrated lensless microscopy module for optical monitoring of adherent growing mammalian cells
Author :
Li, Wei ; Knoll, Thorsten ; Thielecke, Hagen
Author_Institution :
Dept. of Biohybrid Syst., Fraunhofer Inst. for Biomed. Eng., St. Ingbert, Germany
fYear :
2010
fDate :
Aug. 31 2010-Sept. 4 2010
Firstpage :
1012
Lastpage :
1015
Abstract :
Lab-on-a-chip systems are increasingly applied in cell-based assays for toxicology and drug testing. In this paper, an on-chip integrated lensless microscopy module using a direct projection method for optical monitoring of the shadow images of adherent growing mammalian cells is presented. The biological cells are conserved and interfaced by a microfabricated cavity chip with a 1 μm thick silicon nitride (Si3N4) substrate onto the surface of a 5 megapixel CMOS image sensor with 2.2 μm pixel size. The optical resolution of the assembly is estimated by the contact/proximate printing theory from optical lithography. Further characterization is made by imaging microbeads in chips with the Si3N4-membrane as well as in cavity chips with membranes made from dry film resist (DFR, thickness 20, 40 and 60 μm). The module represents a 3 × optical microscope for cell morphology imaging. The function is demonstrated by the growth monitoring of L929 cells cultured in cavity chips with Si3N4 substrate for 2 days and by checking the colorimetric staining of cells with a compromised membrane.
Keywords :
biomedical optical imaging; cellular biophysics; image resolution; optical microscopy; toxicology; L929 cells; Si3N4; cell morphology imaging; cell-based assays; direct projection method; drug testing; integrated lensless microscopy module; lab-on-a-chip; mammalian cells; optical lithography; optical monitoring; toxicology; Biomedical optical imaging; Image resolution; Microscopy; Optical imaging; Optical sensors; Pixel; Animals; Cell Adhesion; Cell Line; Cell Proliferation; Equipment Design; Equipment Failure Analysis; Fibroblasts; Image Enhancement; Lenses; Lighting; Mice; Microfluidic Analytical Techniques; Microscopy; Reproducibility of Results; Semiconductors; Sensitivity and Specificity; Signal Processing, Computer-Assisted; Systems Integration;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2010 Annual International Conference of the IEEE
Conference_Location :
Buenos Aires
ISSN :
1557-170X
Print_ISBN :
978-1-4244-4123-5
Type :
conf
DOI :
10.1109/IEMBS.2010.5627771
Filename :
5627771
Link To Document :
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