Title :
The HVEM signaling pathway in monocytes
Author :
Heo, Sook-Kyoung ; Yoon, Min-A ; Kim, Byung-Sam
Author_Institution :
Dept. of Biomedicine, Univ. of Ulsan, Ulsan
Abstract :
Herpes virus entry mediator (HVEM) is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on many immune cells. In this study we show that treatment of human monocytes with recombinant human LIGHT (rhLIGHT) induces rapid Ca2+ influx in an HVEM-specific manner in parallel with the TNF-alpha production and enhances the bactericidal activities of monocytes. Immunoprecipitation and Western blotting analysis revealed phosphorylation of phospholipase Cgamma1(PLCgamma1) but not PLCgamma2. Silencing PLCgamma1, or pre-incubation of monocytes with PLC inhibitors, antagonists of the inositol-1,4,5-triphosphate receptor or [Ca2+]i inhibitors completely abolished rhLIGHT-induced Ca2+ influx. Our results indicate that Ca2+ is a downstream mediator of the LIGHT/HVEM interaction in monocytes.
Keywords :
biochemistry; bioelectric phenomena; biomembrane transport; cancer; enzymes; microorganisms; molecular biophysics; patient treatment; tumours; HVEM signaling pathway; LIGHT-HVEM interaction; PLC inhibitors; Western blotting analysis; bactericidal activity; calcium influx; herpes virus entry mediator; human monocyte treatment; immune cells; immunoprecipitation analysis; inositol-1,4,5-triphosphate receptor; lymphotoxins; membrane receptors; phosphorylation; recombinant human LIGHT; tumor necrosis factor receptor; Blood; Calcium; Cells (biology); Fluorescence; Humans; Immune system; Inhibitors; Microscopy; Production; Programmable control; cytokine receptor; inflammation; monocyte; signal transduction;
Conference_Titel :
Strategic Technology, 2007. IFOST 2007. International Forum on
Conference_Location :
Ulaanbaatar
Print_ISBN :
978-1-4244-3589-0
Electronic_ISBN :
978-1-4244-1831-2
DOI :
10.1109/IFOST.2007.4798554
