MEMS Coulter counter for dynamic impedance measurement of cells

Author

Wu, Y. ; Almasri, M. ; Benson, J.D.

Author_Institution

Dept. of Electr. & Comput. Eng., Univ. of Missouri, Columbia, MO, USA

fYear

2011

fDate

28-31 Oct. 2011

Firstpage

794

Lastpage

797

Abstract

In this paper, We report the design, modeling, fabrication and characterization MEMS Coulter counter which can monitor the dynamic cell impedance changes in situ as a function of time after mixing isolated cell populations with different extracellular media. The device consists of a multilayer of SU-8 microchannel which is divided into a passive mixing region, a focusing region using negative dielectrophoretic forces, and a measuring region by multiple electroplated vertical electrode pairs. Both fluidic and electrical functionality of the device were tested with using yeast cells in cryoprotectant agent (diluted dimethyl sulfoxide), and microbeads with different dimensions, and dyed fluids. The results demonstrate rapid changes of cell volume within the first 0.6 sec after mixing followed by a stable and a fixed cell volume.

Keywords

biomedical electrodes; dyes; electrophoresis; electroplating; microfluidics; mixing; MEMS coulter counter; SU-8 microchannel multilayer; cell population isolation; cells dynamic impedance measurement; cryoprotectant agent; dyed fluid; dynamic cell impedance monitoring; extracellular media; microbead; multiple electroplated vertical electrode pair; negative dielectrophoretic force; passive mixing region; time 0.6 s; yeast cell; Electrodes; Focusing; Impedance; Impedance measurement; Microchannel; Micromechanical devices; Radiation detectors;

fLanguage

English

Publisher

ieee

Conference_Titel

Sensors, 2011 IEEE

Conference_Location

Limerick

ISSN

1930-0395

Print_ISBN

978-1-4244-9290-9

Type

conf

DOI

10.1109/ICSENS.2011.6127238

Filename

6127238