DocumentCode :
2973944
Title :
Spectra optical detection of biomolecules using a white light source-based spectrophotometric platform
Author :
Cardoso, Susana ; Freitas, Paulo ; Ferreira, Débora ; Minas, Graça ; Miranda, Adelaide
Author_Institution :
INESC-Microsistemas e Nanotecnologias (INESC-MN) & Phys. Dept., IST/Univ. Tec. de Lisboa, Lisbon, Portugal
fYear :
2011
fDate :
28-31 Oct. 2011
Firstpage :
703
Lastpage :
706
Abstract :
This work describes the integration of thin-film optical filters in a lab-on-a-chip device for spectrophotometric analysis of biological fluids using white-light as illumination. Thin-film SiO2/TiO2 multilayers were optimized according to numerical simulations, so that the optical transmittance is tuned for specific wavelengths. These films were deposited on glass substrates by Ion Beam and then integrated in a previously developed lab-on-a-chip platform, which incorporates microfluidic and detection modules. The system operates with a white-light source that is filtered to a narrow spectral band, centred at the desired wavelength, allowing the selective measurement of the light intensity transmitted through the fluid. This intensity is quantified using a colorimetric detection system and then correlated with the biomolecule concentration. Several calcium concentrations in urine were successfully detected in the lab-on-a-chip using the filtering system.
Keywords :
colorimetry; molecular biophysics; optical filters; spectrophotometry; biological fluids; biomolecules; colorimetric detection system; glass substrates; lab-on-a-chip device; spectra optical detection; spectrophotometric analysis; thin-film optical filters; white light source-based spectrophotometric platform; Biomedical optical imaging; Cavity resonators; Molecular biophysics; Optical films; Optical filters; Optical variables measurement;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Sensors, 2011 IEEE
Conference_Location :
Limerick
ISSN :
1930-0395
Print_ISBN :
978-1-4244-9290-9
Type :
conf
DOI :
10.1109/ICSENS.2011.6127366
Filename :
6127366
Link To Document :
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