DocumentCode :
2987091
Title :
All optical histology using femtosecond laser pulses
Author :
Squier, Jeff A. ; Tsai, Philbert S. ; Kleinfeld, D.
Author_Institution :
Dept. of Phys., Colorado Sch. of Mines, Golden, CO, USA
Volume :
3
fYear :
2005
fDate :
22-27 May 2005
Firstpage :
2037
Abstract :
We discuss the use of femtosecond laser pulses for serial histology. Successive iterations of multiphoton imaging and ablation provide diffraction-limited volumetric data that is used to reconstruct the architectonics of labeled cells or microvasculature.
Keywords :
biological tissues; biomedical optical imaging; cellular biophysics; high-speed optical techniques; laser applications in medicine; light diffraction; multiphoton processes; ablation; architectonics; cells; diffraction-limited volumetric data; femtosecond laser pulses; microvasculature; multiphoton imaging; optical histology; serial histology; Biomedical optical imaging; Fluorescence; Laser ablation; Lasers and electrooptics; Optical pulses; Optical scattering; Proteins; Surface emitting lasers; Surface reconstruction; Ultrafast optics;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Lasers and Electro-Optics, 2005. (CLEO). Conference on
Print_ISBN :
1-55752-795-4
Type :
conf
DOI :
10.1109/CLEO.2005.202360
Filename :
1573426
Link To Document :
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