DocumentCode
2987091
Title
All optical histology using femtosecond laser pulses
Author
Squier, Jeff A. ; Tsai, Philbert S. ; Kleinfeld, D.
Author_Institution
Dept. of Phys., Colorado Sch. of Mines, Golden, CO, USA
Volume
3
fYear
2005
fDate
22-27 May 2005
Firstpage
2037
Abstract
We discuss the use of femtosecond laser pulses for serial histology. Successive iterations of multiphoton imaging and ablation provide diffraction-limited volumetric data that is used to reconstruct the architectonics of labeled cells or microvasculature.
Keywords
biological tissues; biomedical optical imaging; cellular biophysics; high-speed optical techniques; laser applications in medicine; light diffraction; multiphoton processes; ablation; architectonics; cells; diffraction-limited volumetric data; femtosecond laser pulses; microvasculature; multiphoton imaging; optical histology; serial histology; Biomedical optical imaging; Fluorescence; Laser ablation; Lasers and electrooptics; Optical pulses; Optical scattering; Proteins; Surface emitting lasers; Surface reconstruction; Ultrafast optics;
fLanguage
English
Publisher
ieee
Conference_Titel
Lasers and Electro-Optics, 2005. (CLEO). Conference on
Print_ISBN
1-55752-795-4
Type
conf
DOI
10.1109/CLEO.2005.202360
Filename
1573426
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