Title :
All optical histology using femtosecond laser pulses
Author :
Squier, Jeff A. ; Tsai, Philbert S. ; Kleinfeld, D.
Author_Institution :
Dept. of Phys., Colorado Sch. of Mines, Golden, CO, USA
Abstract :
We discuss the use of femtosecond laser pulses for serial histology. Successive iterations of multiphoton imaging and ablation provide diffraction-limited volumetric data that is used to reconstruct the architectonics of labeled cells or microvasculature.
Keywords :
biological tissues; biomedical optical imaging; cellular biophysics; high-speed optical techniques; laser applications in medicine; light diffraction; multiphoton processes; ablation; architectonics; cells; diffraction-limited volumetric data; femtosecond laser pulses; microvasculature; multiphoton imaging; optical histology; serial histology; Biomedical optical imaging; Fluorescence; Laser ablation; Lasers and electrooptics; Optical pulses; Optical scattering; Proteins; Surface emitting lasers; Surface reconstruction; Ultrafast optics;
Conference_Titel :
Lasers and Electro-Optics, 2005. (CLEO). Conference on
Print_ISBN :
1-55752-795-4
DOI :
10.1109/CLEO.2005.202360
