Title :
Laser-assisted controlled micro-injection into living cells
Author :
Peng, Cheng ; Wilke, Ingrid ; Palazzo, Robert E.
Author_Institution :
Dept. of Phys., Rensselaer Polytech. Inst., Troy, NY, USA
Abstract :
A titanium-sapphire laser emitting 100 fs short laser pulses at 800 nm wavelength is employed to inject an impermeable dye into single living cells. The cell morphology remains intact after this process. A new method to analyze dye uptake and evaluate membrane integrity is suggested.
Keywords :
biomembranes; cellular biophysics; dyes; high-speed optical techniques; laser applications in medicine; solid lasers; titanium; 100 fs; 800 nm; Al2O3:Ti; cell morphology; dye uptake; impermeable dye; laser-assisted controlled micro-injection; living cells; membrane integrity; short laser pulses; titanium-sapphire laser; Biomedical optical imaging; Biomembranes; Cells (biology); Fluorescence; Laser theory; Microinjection; Optical control; Optical microscopy; Optical pulses; Ultrafast optics;
Conference_Titel :
Lasers and Electro-Optics, 2005. (CLEO). Conference on
Print_ISBN :
1-55752-795-4
DOI :
10.1109/CLEO.2005.202452
