Title :
Nanometer axial position sensing in optical tweezers using differential confocal microscopy
Author :
Kuo, Kai-Wen ; Xiao, Jian-Long ; Lee, Chau-Hwang
Author_Institution :
Inst. of Biophotonics Eng., Nat. Yang-Ming Univ., Taipei, Taiwan
Abstract :
We use differential confocal microscopy for axial detection in optical tweezers. The fluctuation of a 3 μm bead is about 10 nm at 16 mW. This technique is employed for studying protein elasticity and microrheology.
Keywords :
biological techniques; biomechanics; biorheology; elasticity; molecular biophysics; optical microscopy; proteins; radiation pressure; bead fluctuation; differential confocal microscopy; microrheology; nanometer axial position sensing; optical tweezers; protein elasticity; Biomedical optical imaging; Fluctuations; Fluorescence; Laser beams; Lasers and electrooptics; Optical microscopy; Optical scattering; Optical sensors; Proteins; Semiconductor lasers;
Conference_Titel :
Lasers and Electro-Optics, 2005. (CLEO). Conference on
Print_ISBN :
1-55752-795-4
DOI :
10.1109/CLEO.2005.202455
