Title :
Toward detection of harmful algae blooms by in situ surface plasmon resonance spectroscopy
Author :
Laurent, Sébastien ; Colas, Florent ; Hamelin, Muriel ; Crassous, Marie-Pierre ; Antoine, Elisabeth ; Lehaitre, Michel ; Compere, Chantal
Author_Institution :
ERT / Interfaces & Capteurs, IFREMER, Plouzane
Abstract :
Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal seawater is of great economical importance for marine resources exploitation. Here, we propose a rapid test for the detection of A. minutum by surface plasmon resonance spectroscopy. First, whole genomic DNA is extracted from the algae. Second, a 677 bp long portion of the 28S ribosomal DNA is amplified by PCR. Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate.
Keywords :
DNA; biological techniques; biosensors; genomics; gold; health hazards; microorganisms; surface plasmon resonance; toxicology; 28S ribosomal DNA; Alexandrium minutum; DNA functionalized gold substrate; PCR; algae genomic DNA; coastal seawater; harmful algae bloom detection; marine resources exploitation; paralytic shellfish poisoning; phycotoxin; phytoplankton; polymerase chain reaction; surface plasmon resonance spectroscopy; Algae; DNA; Genomics; Humans; Monitoring; Plasmons; Resonance; Sea measurements; Spectroscopy; Testing; PCR; algal detection; in situ; spectroscopy; surface plasmon resonance;
Conference_Titel :
Sensors Applications Symposium, 2009. SAS 2009. IEEE
Conference_Location :
New Orleans, LA
Print_ISBN :
978-1-4244-2786-4
Electronic_ISBN :
978-1-4244-2787-1
DOI :
10.1109/SAS.2009.4801771