Ultrasound-mediated gene delivery into hard-to-transfect KG-1 cells

Author

Wong, Peter ; Choi, Michael A. ; Gul-Uludag, Hilal ; Ang, Woon T. ; Xu, Peng ; Xing, James ; Chen, Jie

Author_Institution

Dept. of Biomed. Eng., Univ. of Alberta, Edmonton, AB, Canada

fYear

2011

fDate

7-8 April 2011

Firstpage

143

Lastpage

146

Abstract

In this paper, we explore the possibility of increasing the transfection rate of hard-to-transfect cells (such as KG-1 cells) using ultrasound-mediated gene delivery (USD). Single application ultrasound induced as well as the synergistic properties of ultrasound with commonly used transfection reagents and repeated sessions USD were investigated and evaluated using GFP plasmid. KG-1 cells were not transfected by combining a commonly used transfection reagent and USD. However, repeated ultrasound-mediated treatments were able to increase transfection rate to 15% with a viability of 97%, as compared to 2% with the transfection reagent, Lipofectamine, alone.

Keywords

biomedical ultrasonics; cellular biophysics; gene therapy; patient treatment; GFP plasmid; Lipofectamine; hard-to-transfect KG-1 cells; transfection rate; ultrasound-mediated gene delivery; viability; Acoustics; Conferences; Fluorescence; Gene therapy; Stem cells; Suspensions; Ultrasonic imaging;

fLanguage

English

Publisher

ieee

Conference_Titel

Life Science Systems and Applications Workshop (LiSSA), 2011 IEEE/NIH

Conference_Location

Bethesda, MD

Print_ISBN

978-1-4577-0421-5

Type

conf

DOI

10.1109/LISSA.2011.5754179

Filename

5754179