Title :
Characterizing neuropathy in the sciatic nerve of a poly I:C - induced rat model of type I diabetes
Author :
League-Pike, W.F. ; Mason, D.L. ; Shonat, Ross D.
Author_Institution :
Worcester Polytech. Inst., MA, USA
Abstract :
As part of a larger study to investigate the complications of diabetes, this study was aimed at developing the materials and methods for measuring nerve conduction velocity in the rat sciatic nerve. Using a specially designed nerve conduction velocity chamber, we measured action potential waveforms in an ex vivo sciatic nerve preparation at two locations along the nerve following upstream electrical stimulation. Conduction velocity in a non-diabetic control rat was 39 ± 5 m/s and decreased by 88% following mechanical crushing of the nerve. With an operational ex vivo nerve conduction velocity chamber, we will apply it to the measurement of nerve conduction velocity in a poly I:C - induced rat model of type 1 diabetes.
Keywords :
bioelectric potentials; biomedical measurement; diseases; neurophysiology; Polyinosinic:Polycytidilic acid; action potential waveforms; ex vivo nerve conduction velocity chamber; ex vivo sciatic nerve preparation; nerve conduction velocity; neuropathy characterization; poly I:C - induced rat model; sciatic nerve; type I diabetes; upstream electrical stimulation; Animals; Biomedical engineering; Biomedical measurements; Conducting materials; Diabetes; Electric variables measurement; Electrical stimulation; Insulin; Integrated circuit modeling; Velocity measurement;
Conference_Titel :
Bioengineering Conference, 2004. Proceedings of the IEEE 30th Annual Northeast
Print_ISBN :
0-7803-8285-4
DOI :
10.1109/NEBC.2004.1300088