Title :
Effect of tissue factor on plasma and packed RBC clotting parameters
Author :
Patel, Darshana R. ; Davidson, John ; Kristol, David ; Spillert, Charles R.
Author_Institution :
New Jersey Inst. of Technol., Newark, NJ, USA
Abstract :
Tissue factor (TF) is a transmembrane glycoprotein and the main triggering element for blood coagulation. Expression of TF on the cell surfaces and its appearance as a soluble molecule are characteristic features of acute and chronic inflammation in conditions such as arteriosclerosis leading to stroke and myocardial infection. A study was performed on human citrated whole blood, by separating plasma from the red blood cells. Ten microlitres of 25% tissue factor (TF) was added to both the plasma and packed red blood cells (RBC´s), and the clotting time, rate of clot formation and the maximum strength of the clot determined. TF bearing samples clotted more rapidly and had greater clot strength when compared to that of control aliquots.
Keywords :
biomembranes; blood; cellular biophysics; coagulation; diseases; molecular biophysics; proteins; acute inflammation; arteriosclerosis; blood coagulation; cell surfaces; chronic inflammation; clot formation; clot strength; clotting time; human citrated whole blood; myocardial infection; packed RBC clotting parameters; plasma; red blood cells; stroke; tissue factor effect; transmembrane glycoprotein; Cloning; Coagulation; Humans; Myocardium; Plasma displays; Plasma materials processing; Plasma properties; Red blood cells; Testing; Viscosity;
Conference_Titel :
Bioengineering Conference, 2004. Proceedings of the IEEE 30th Annual Northeast
Print_ISBN :
0-7803-8285-4
DOI :
10.1109/NEBC.2004.1300089
