Title :
Biochemical IC chip toward cell free DNA protein synthesis
Author :
Ikuta, Koji ; Maruo, Shoji ; Fukaya, Yuichiro ; Fujisawa, Tetsuya
Author_Institution :
Dept. of Micro Syst. Eng., Nagoya Univ., Japan
Abstract :
The several type of biochemical IC chips with photo detective micro reactor has been developed. The real time monitoring and controlling cell free synthesis of protein was demonstrated successfully. The photo protein of firefly so called Luciferase was synthesized. The biochemical luminescence was monitored by built-in photo diode in the micro reactor to transfer reaction status into voltage signal. Feedback control synthetic reaction was verified experimentally. The results achieved here should be key technology for future DNA and RNA synthesis without biological cell
Keywords :
DNA; biocontrol; biological techniques; chemical technology; elemental semiconductors; feedback; integrated circuit technology; micromechanical devices; proteins; silicon; Luciferase; RNA synthesis; Si; biochemical IC chip; biochemical luminescence; built-in photo diode; cell free DNA protein synthesis; feedback control synthetic reaction; micro reactor; photo detective micro reactor; protein; reaction status; real time monitoring; voltage signal; DNA; Diodes; Feedback control; Inductors; Integrated circuit synthesis; Luminescence; Monitoring; Proteins; Signal synthesis; Voltage;
Conference_Titel :
Micro Electro Mechanical Systems, 1998. MEMS 98. Proceedings., The Eleventh Annual International Workshop on
Conference_Location :
Heidelberg
Print_ISBN :
0-7803-4412-X
DOI :
10.1109/MEMSYS.1998.659742
