Title :
Multiphoton Fluorescence Imaging of NADH to Investigate Metabolic Changes in Human Epileptic Tissue in vitro
Author :
Chia, Thomas H. ; Williamson, Anne ; Spencer, Dennis D. ; Levene, Michael J.
Author_Institution :
Yale Univ., New Haven
Abstract :
NADH is an intrinsic fluorophore that provides cellular metabolic information. Abnormal neuronal-astrocytic metabolic coupling is hypothesized in temporal lobe epilepsy. We investigate metabolic pathologies in human and rodent epilepsy models using multiphoton microscopy of NADH.
Keywords :
biochemistry; biological tissues; biomedical optical imaging; brain; cellular biophysics; diseases; enzymes; fluorescence; molecular biophysics; multiphoton processes; neurophysiology; optical microscopy; NADH; abnormal neuronal-astrocytic metabolic coupling mechanism; cellular metabolic information; human epileptic tissue; in vitro analysis; intrinsic fluorophore; metabolic pathologies; multiphoton fluorescence imaging; multiphoton microscopy; nicotinamide adenine dinucleotide; rodent epilepsy models; temporal lobe epilepsy; Biochemistry; Calcium; Epilepsy; Fluorescence; Hippocampus; Humans; In vitro; Optical imaging; Optical microscopy; Temporal lobe;
Conference_Titel :
Lasers and Electro-Optics, 2007. CLEO 2007. Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-55752-834-6
DOI :
10.1109/CLEO.2007.4453056
