Title :
Enhanced Two-Photon In Vivo Flow Cytometry with an Extended Cavity Laser
Author :
Tkaczyk, Eric R. ; Ye, Jing Yong ; Katnik, Steve ; Luker, Kathryn E. ; Luker, Gary D. ; Baker, James R. ; Norris, Theodore B.
Author_Institution :
Univ. of Michigan, Ann Arbor
Abstract :
We use multiphoton in vivo flow cytometry to monitor multiple cell lines. With a reduced-repetition-rate (20-MHz) mode-locked oscillator, the signal is enhanced four-fold, enabling detection in blood of multiple cell lines expressing different GFP variants.
Keywords :
bio-optics; blood flow measurement; cellular biophysics; laser cavity resonators; laser mode locking; two-photon processes; blood detection; extended cavity laser; frequency 20 MHz; mode-locked oscillator; multiphoton in vivo flow cytometry; multiple cell lines; two-photon in vivo flow cytometry; Biomedical monitoring; Biomedical optical imaging; Blood; Cells (biology); Fluorescence; In vivo; Laser excitation; Laser transitions; Power lasers; Ultrafast optics;
Conference_Titel :
Lasers and Electro-Optics, 2007. CLEO 2007. Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-55752-834-6
DOI :
10.1109/CLEO.2007.4453062
