Title :
Imaging tools for the analysis of microvessel and angiogenesis development in a tridimensional cell culture system
Author_Institution :
Dept. of Biomech. & Biomed. Mater., Univ. Teknol. Malaysia, Johor Bahru, Malaysia
Abstract :
This study presents the design and validation of imaging tools to access and analyses the development of microvessel and angiogenesis inside a tridimensional (3D) cell culture system. Angiogenesis is a key mechanism during normal development as well as in cancer cell growth. Phase contrast, fluorescence, and confocal microscopy were used to determine the cell attachment, tube-like structure formation and other cellular events inside our 3D system.
Keywords :
biomedical imaging; blood vessels; cancer; cellular biophysics; fluorescence; optical microscopy; 3D cell culture system; angiogenesis; cancer cell growth; cell attachment; confocal microscopy; fluorescence; imaging tools; microvessel; phase contrast; Fluorescence; angiogenesis; confocal microscopy; human umbilical vein endothelial (HUVEC) cell; microvessel; tridimensinal (3D) system;
Conference_Titel :
Instrumentation Control and Automation (ICA), 2011 2nd International Conference on
Conference_Location :
Bandung
Print_ISBN :
978-1-4577-1462-7
DOI :
10.1109/ICA.2011.6130152
