Fabrication and research of flexible arrayed glucose biosensor combined with the magnetic beads

In this study, the ruthenium dioxide (RuO₂) sensing films were prepared by radio frequency sputtering system on the flexible polyethylene terephthalate (PET) and used the screen printer technology to prepare the conducting wires to connect the sensing windows and spread on insulation layer by epoxy which can protect the conducting wires and sensing windows. In the experimental, first, the biosensors were analyzed for the pH value of the hysteresis reaction (5.651 mV). Second, the magnetic beads and glucose oxidase (GOD) were combined with covalent binding, and coated on the sensing windows by Nafion. It was used to measure the linearity and sensitivity, as well as analyzed the different volume ratios of Nafion and GOD and the stability of the glucose biosensors. The results showed that the glucose biosensors exhibit a high linearity (0.987), sensitivity (29.652 mV/mM) by the volume ratio of Nafion to GOD is 3:4 and present high stability, which can be measured the glucose solution, repeatedly.