Title :
Single Molecule Pulsed Interleaved Excitation Fluorescence Resonance Energy Transfer (PIE-FRET) inside Nanometer-scale Apertures at Biologically Relevant Concentration
Author :
Fore, Samantha ; Huser, Thomas ; Yuen, Yin ; Hesselink, Lambertus
Author_Institution :
Univ. of California Davis, Sacramento
Abstract :
PIE-FRET offers significant advantages over conventional single molecule FRET techniques, but it still requires the dilution of samples to biologically low concentrations. Here, we present FRET measurements inside nm-sized apertures at -1000 times higher concentration.
Keywords :
DNA; fluorescence; molecular biophysics; DNA duplex; biological interactions; biologically relevant concentration; pulsed interleaved excitation fluorescence resonance energy transfer; single molecule PIE-FRET; Apertures; Autocorrelation; Biological information theory; Energy exchange; Fluorescence; Nanobioscience; Optical microscopy; Optical surface waves; Resonance; Testing;
Conference_Titel :
Lasers and Electro-Optics, 2007. CLEO 2007. Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-55752-834-6
DOI :
10.1109/CLEO.2007.4453191
