• DocumentCode
    3015564
  • Title

    Single Molecule Pulsed Interleaved Excitation Fluorescence Resonance Energy Transfer (PIE-FRET) inside Nanometer-scale Apertures at Biologically Relevant Concentration

  • Author

    Fore, Samantha ; Huser, Thomas ; Yuen, Yin ; Hesselink, Lambertus

  • Author_Institution
    Univ. of California Davis, Sacramento
  • fYear
    2007
  • fDate
    6-11 May 2007
  • Firstpage
    1
  • Lastpage
    2
  • Abstract
    PIE-FRET offers significant advantages over conventional single molecule FRET techniques, but it still requires the dilution of samples to biologically low concentrations. Here, we present FRET measurements inside nm-sized apertures at -1000 times higher concentration.
  • Keywords
    DNA; fluorescence; molecular biophysics; DNA duplex; biological interactions; biologically relevant concentration; pulsed interleaved excitation fluorescence resonance energy transfer; single molecule PIE-FRET; Apertures; Autocorrelation; Biological information theory; Energy exchange; Fluorescence; Nanobioscience; Optical microscopy; Optical surface waves; Resonance; Testing;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Lasers and Electro-Optics, 2007. CLEO 2007. Conference on
  • Conference_Location
    Baltimore, MD
  • Print_ISBN
    978-1-55752-834-6
  • Type

    conf

  • DOI
    10.1109/CLEO.2007.4453191
  • Filename
    4453191