DocumentCode
3015564
Title
Single Molecule Pulsed Interleaved Excitation Fluorescence Resonance Energy Transfer (PIE-FRET) inside Nanometer-scale Apertures at Biologically Relevant Concentration
Author
Fore, Samantha ; Huser, Thomas ; Yuen, Yin ; Hesselink, Lambertus
Author_Institution
Univ. of California Davis, Sacramento
fYear
2007
fDate
6-11 May 2007
Firstpage
1
Lastpage
2
Abstract
PIE-FRET offers significant advantages over conventional single molecule FRET techniques, but it still requires the dilution of samples to biologically low concentrations. Here, we present FRET measurements inside nm-sized apertures at -1000 times higher concentration.
Keywords
DNA; fluorescence; molecular biophysics; DNA duplex; biological interactions; biologically relevant concentration; pulsed interleaved excitation fluorescence resonance energy transfer; single molecule PIE-FRET; Apertures; Autocorrelation; Biological information theory; Energy exchange; Fluorescence; Nanobioscience; Optical microscopy; Optical surface waves; Resonance; Testing;
fLanguage
English
Publisher
ieee
Conference_Titel
Lasers and Electro-Optics, 2007. CLEO 2007. Conference on
Conference_Location
Baltimore, MD
Print_ISBN
978-1-55752-834-6
Type
conf
DOI
10.1109/CLEO.2007.4453191
Filename
4453191
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