DocumentCode :
3015689
Title :
Artificially induced cell fusion by optical tweezers manipulation
Author :
Shuxun Chen ; Xiaolin Wang ; Jinping Cheng ; Chi-wing Kong ; Shuk Han Cheng ; Li, Ronald A. ; Dong Sun
Author_Institution :
Dept. of Mech. & Biomed. Eng., City Univ. of Hong Kong, Hong Kong, China
fYear :
2013
fDate :
5-8 Aug. 2013
Firstpage :
333
Lastpage :
336
Abstract :
Artificially induced cell fusion is becoming a powerful tool in developmental biology. In this paper, we verify the feasibility of an engineering laser-induced solution to achieve precise cell fusion at the single-cell level. Two cells formed a cell pair with the manipulation of optical tweezers and fused after irradiation by pulsed ultraviolet laser. Time-lapse imaging of the cells during fusion and cytoplasmic fluorescent protein transfer between the cells indicated successful cell fusion. After fusion, a binucleate cell was created when the two nuclei approached each other. The mitosis of the fused cell during continuous culture verified the viability of the cell. This artificially induced cell fusion technique provides a new opportunity for studying fusion phenomenon at the molecular and nano-scale precision levels.
Keywords :
biochemistry; biological techniques; cellular transport; fluorescence; molecular biophysics; proteins; radiation pressure; artificially induced cell fusion technique; binucleate cell; cell mitosis; cell pair formation; cell viability; cytoplasmic fluorescent protein transfer; developmental biology; molecular levels; nanoscale precision levels; optical tweezer manipulation; pulsed ultraviolet laser; time-lapse cell imaging; Biomedical optical imaging; Laser ablation; Laser beam cutting; Laser beams; Laser fusion; Measurement by laser beam; Surface emitting lasers;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Nanotechnology (IEEE-NANO), 2013 13th IEEE Conference on
Conference_Location :
Beijing
ISSN :
1944-9399
Print_ISBN :
978-1-4799-0675-8
Type :
conf
DOI :
10.1109/NANO.2013.6720868
Filename :
6720868
Link To Document :
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