Title :
Micropatterning of bioactive ligands for high-throughput study neuronal network functions
Author :
Li Wei ; Xu Zhen ; Peng Shi
Author_Institution :
Dept. of Mech. & Biomed. Eng., City Univ. of Hong Kong, Hong Kong, China
Abstract :
Synapses are essential components for signal processing and transmission in the nervous system, whose function is affected in many brain diseases and disorders. We demonstrated the successful control of synapse formation by micropatterning of synaptogenic protein, neuroligin (NL). Interestingly, the presence of a hydrophobic GPI moiety, promoted NL´s biological activity. Also, the micropatterns of synaptogenic factors were included in a neurite growth/guidance platform, which could directly be used as a high-throughput platform for study different aspects in the development and dynamics of neuronal network.
Keywords :
biochemistry; bioelectric potentials; brain; cellular biophysics; hydrophobicity; molecular biophysics; neural nets; neurophysiology; proteins; bioactive ligand micropatterning; brain diseases; brain disorders; high-throughput platform; hydrophobic GPI moiety; nervous system; neurite growth platform; neurite guidance platform; neuroligin biological activity; neuroligin micropatterning; neuronal network functions; signal processing; signal transmission; synapse formation control; synaptogenic protein micropatterning; Biological neural networks; Nerve fibers; Phase locked loops; Protein engineering; Proteins; Substrates;
Conference_Titel :
Nanotechnology (IEEE-NANO), 2013 13th IEEE Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4799-0675-8
DOI :
10.1109/NANO.2013.6720879
