Inertial microfluidics for circulating tumor cell separation and detection

Author

Xingyu Jiang

Author_Institution

Nat. Center for Nanosci. & Technol., Beijing, China

fYear

2013

fDate

5-8 Aug. 2013

Firstpage

488

Lastpage

491

Abstract

This report demonstrates a passive double spiral microfluidic device allowing rapid and label-free tumor cell separation by inertial microfluidics. Results from separating tumor cells spiked into whole blood indicate a separation efficiency over 90% at the throughput of 3.33×107 cells/min. The separated tumor cells are trapped and enriched on an integrated polycarbonate filter located on top of the outlet of microchannels. Nucleic acids from MCF-7 cells are detected by loop-mediated isothermal amplification (LAMP).

Keywords

bioMEMS; biomedical measurement; cellular transport; haemodynamics; macromolecules; microchannel flow; molecular biophysics; patient diagnosis; separation; tumours; LAMP; MCF-7 cells; circulating tumor cell detection; circulating tumor cell separation; inertial microfluidics; integrated polycarbonate filter; label-free tumor cell separation; loop-mediated isothermal amplification; microchannel outlet; nucleic acids; rapid tumor cell separation; separation efficiency; spiral microfluidic device; whole blood; Blood; Cancer; Cells (biology); Microchannel; Microfluidics; Spirals; Tumors;

fLanguage

English

Publisher

ieee

Conference_Titel

Nanotechnology (IEEE-NANO), 2013 13th IEEE Conference on

Conference_Location

Beijing

ISSN

1944-9399

Print_ISBN

978-1-4799-0675-8

Type

conf

DOI

10.1109/NANO.2013.6720890

Filename

6720890