A novel shape information based spindle positioning method with the polarized light microscopy

Author

Zheng Wang ; Guodong Chen ; Haibo Huang ; Ping Gao ; Zhenhua Wang ; Dong Sun ; Lining Sun

Author_Institution

Inst. of Neurosci., Soochow Univ., Suzhou, China

fYear

2013

fDate

5-8 Aug. 2013

Firstpage

150

Lastpage

153

Abstract

Cell structures analyze and cell organelles play important roles in bio-manipulation. However, it is difficult to image the live cell organelles without damage by using the common microscopy. Polarized light microscopy provides unique opportunities for analyzing the architectural dynamics of living cells, tissues, and whole organisms. In this paper, a novel cell spindle positioning method with the polarized light is introduced. Firstly, the live cell spindle apparatus structure imaging method with the polarized light is presented; secondly, a novel shape information based spindle positioning method is introduced; then, cell spindle positioning experiments are carried out, experimental results shows the proposed method is valid..

Keywords

biological techniques; biological tissues; biology computing; cellular biophysics; image processing; light polarisation; optical microscopy; biomanipulation; cell organelles; cell spindle positioning method; cell structures; common microscopy; live cell organelle imaging; live cell spindle apparatus structure imaging method; living cell architectural dynamics; polarized light microscopy; shape information; tissue architectural dynamics; whole organism architectural dynamics; Image edge detection; Microscopy; Object detection; Shape; Sun; Vectors;

fLanguage

English

Publisher

ieee

Conference_Titel

Nanotechnology (IEEE-NANO), 2013 13th IEEE Conference on

Conference_Location

Beijing

ISSN

1944-9399

Print_ISBN

978-1-4799-0675-8

Type

conf

DOI

10.1109/NANO.2013.6721013

Filename

6721013