Title :
Detection of Bacillus thuringiensis Spore Germination via CaDPA Biomarker Using Laser Tweezers Raman Spectroscopy
Author :
Huang, Shu-shi ; De Chen ; Li, Yong-qing
Author_Institution :
East Carolina Univ., Greenville
Abstract :
We report real-time observation of nutrient-trigged germination process of single Bacillus thuringiensis spores by detection of CaDPA biomarker using laser tweezers Raman spectroscopy. The time-to-germination was heterogeneous for spores and the distribution was measured.
Keywords :
Raman spectra; biological techniques; cellular biophysics; laser beam applications; microorganisms; radiation pressure; Bacillus thuringiensis; CaDPA biomarker; calcium dipicolinate; laser tweezers Raman spectroscopy; nutrient-trigged germination; spore germination; Atomic force microscopy; Biomarkers; Biomedical optical imaging; Fungi; Kinetic theory; Laser beams; Monitoring; Optical refraction; Raman scattering; Spectroscopy;
Conference_Titel :
Lasers and Electro-Optics, 2007. CLEO 2007. Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-55752-834-6
DOI :
10.1109/CLEO.2007.4453496
