Title :
A micro cell lysis device
Author :
Lee, S.W. ; Yowanto, H. ; Tai, Y.C.
Author_Institution :
Caltech Macromaching Lab., California Inst. of Technol., Pasadena, CA, USA
Abstract :
A new micromachined cell lysis device is developed. It is designed for miniature bio-analysis systems where cell lysing is needed to obtain intracellular materials for further analysis such as DNA identification. It consists of multi-electrode pairs to apply electric fields to cells. We adopt the means of using electric field lysing because it call greatly simplify purification steps for preparation of biological samples, when compared to conventional chemical methods. Yeast, Chinese cabbage, radish cells and E. Coli are tested with the device. The lysis of yeast, Chinese cabbage, radish cells is observed by a microscope. The experimental observation suggests E. Coli are also lysed by the pulsed electric field. The range of electric field for the lysis is on the order of 1 kV/cm to 10 kV/cm. In addition, for practical reasons, we reduce the voltage required for lysing to less than 10 V by making the electrode gap on the order of microns
Keywords :
DNA; biological techniques; biomembranes; cellular biophysics; electrochemical analysis; micromechanical devices; 10 V; Chinese cabbage; DNA identification; E. Coli; Yeast; biological sample; chemical methods; electric fields; electrode gap; intracellular materials; micro cell lysis device; micromachined cell lysis device; miniature bio-analysis; multi-electrode pairs; pulsed electric field; radish cells; Biological materials; Biomembranes; Cells (biology); Chemicals; DNA; Electrodes; Fungi; Purification; RNA; Voltage;
Conference_Titel :
Micro Electro Mechanical Systems, 1998. MEMS 98. Proceedings., The Eleventh Annual International Workshop on
Conference_Location :
Heidelberg
Print_ISBN :
0-7803-4412-X
DOI :
10.1109/MEMSYS.1998.659798
