Title :
High resolution chemical mapping of biomimetic membranes by force volume imaging
Author :
Nussio, Matthew R. ; Voelcker, Nicolas H. ; Sykes, Matthew J. ; Flavel, B.S. ; Miners, John O. ; Shapter, Joseph G.
Author_Institution :
Sch. of Chem., Phys. & Earth Sci., Flinders Univ., Adelaide, SA
Abstract :
The analysis of membrane domain segregation and phase separation is of significant interest for the understanding of cell membranes. In this paper, we report a new approach to atomic force microscopy (AFM) of artificial membranes, using both functionalised AFM tips and ldquoForce Volumerdquo AFM imaging for the analysis of membrane phase separation. Simultaneous topology and mapping of interaction forces of binary component phospholipid bilayer membranes was performed yielding novel insights into membrane structure at unprecedented resolution.
Keywords :
atomic force microscopy; biomimetics; lipid bilayers; phase separation; segregation; AFM; atomic force microscopy; biomimetic membranes; force volume imaging; high resolution chemical mapping; membrane domain segregation; phase separation; phospholipid bilayer membranes; Atomic force microscopy; Biomembranes; Biomimetics; Cells (biology); Chemicals; High-resolution imaging; Image analysis; Image resolution; Simultaneous localization and mapping; Topology; force-volume; microscopy; phospholipids;
Conference_Titel :
Nanoscience and Nanotechnology, 2008. ICONN 2008. International Conference on
Conference_Location :
Melbourne, Vic.
Print_ISBN :
978-1-4244-1503-8
Electronic_ISBN :
978-1-4244-1504-5
DOI :
10.1109/ICONN.2008.4639261
