High resolution chemical mapping of biomimetic membranes by force volume imaging

Author

Nussio, Matthew R. ; Voelcker, Nicolas H. ; Sykes, Matthew J. ; Flavel, B.S. ; Miners, John O. ; Shapter, Joseph G.

Author_Institution

Sch. of Chem., Phys. & Earth Sci., Flinders Univ., Adelaide, SA

fYear

2008

fDate

25-29 Feb. 2008

Firstpage

121

Lastpage

124

Abstract

The analysis of membrane domain segregation and phase separation is of significant interest for the understanding of cell membranes. In this paper, we report a new approach to atomic force microscopy (AFM) of artificial membranes, using both functionalised AFM tips and ldquoForce Volumerdquo AFM imaging for the analysis of membrane phase separation. Simultaneous topology and mapping of interaction forces of binary component phospholipid bilayer membranes was performed yielding novel insights into membrane structure at unprecedented resolution.

Keywords

atomic force microscopy; biomimetics; lipid bilayers; phase separation; segregation; AFM; atomic force microscopy; biomimetic membranes; force volume imaging; high resolution chemical mapping; membrane domain segregation; phase separation; phospholipid bilayer membranes; Atomic force microscopy; Biomembranes; Biomimetics; Cells (biology); Chemicals; High-resolution imaging; Image analysis; Image resolution; Simultaneous localization and mapping; Topology; force-volume; microscopy; phospholipids;

fLanguage

English

Publisher

ieee

Conference_Titel

Nanoscience and Nanotechnology, 2008. ICONN 2008. International Conference on

Conference_Location

Melbourne, Vic.

Print_ISBN

978-1-4244-1503-8

Electronic_ISBN

978-1-4244-1504-5

Type

conf

DOI

10.1109/ICONN.2008.4639261

Filename

4639261