Label-Free Continuous Micro Cell Sorter with Antibody-Immobilized Oblique Grooves

Author

Hashimoto, Shin-ichi ; Nishimura, Takahiro ; Miwa, Junichi ; Suzuki, Yuji ; Kasagi, Nobuhide

Author_Institution

Dept. of Mech. Eng., The Univ. of Tokyo, Tokyo

fYear

2009

fDate

25-29 Jan. 2009

Firstpage

60

Lastpage

63

Abstract

We report development of a novel label-free continuous cell separation method for the extraction of rare cells from a limited amount of sample. The separation method is based on specific adhesion between target cells and antibody immobilized on oblique micro grooves etched into the channel wall. Due to asymmetric adhesive force, cross-flow displacement of the target cells is induced. Label-free lateral separation of micro beads as the cell model has been successfully achieved. Cross-flow displacement of human umbilical-vein endothelial cells (HUVEC) with the CD31 antibody is also demonstrated.

Keywords

adhesion; bioMEMS; biological techniques; cellular biophysics; molecular biophysics; proteins; separation; CD31 antibody; adhesion; cell model; continuous cell separation; human umbilical-vein endothelial cells; microbeads; microcell sorter; Adhesives; Bonding; Etching; Fluorescence; Humans; Labeling; Magnetic separation; Silicon; Sorting; Stem cells;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems, 2009. MEMS 2009. IEEE 22nd International Conference on

Conference_Location

Sorrento

ISSN

1084-6999

Print_ISBN

978-1-4244-2977-6

Electronic_ISBN

1084-6999

Type

conf

DOI

10.1109/MEMSYS.2009.4805319

Filename

4805319