Title :
Flow-Lysometry and its Biomedical Application: Cytosolic Analysis of Single Cells in Large Populations
Author :
Lee, W.C. ; Kuypers, F.A. ; Cho, Y.-H. ; Pisano, A.P.
Author_Institution :
Red Cell Membrane Lab., Children´´s Hosp. Oakland Res. Inst., CA
Abstract :
We present a novel single-cell analyzer, flow-lysometry, to measure cytosolic components in large cell populations. The present flow-lysometry performs and synchronizes three functions (cell-detection, cell-lysis, and component-sensing) in a continuous microfluidic channel, thus achieving high-throughput measurements (20 cells/min) of wide target components. In the experimental study, we measure the cytosolic component (Ca++ ion) from each single RBC and verify population analysis of single cells in mixed cell populations. Thus, this work shows that the present flow-lysometry is useful to characterize complex cell populations, which is required for various biomedical studies.
Keywords :
bioMEMS; bioelectric phenomena; biomedical measurement; blood; cellular biophysics; microfluidics; microsensors; biomedical application; cell detection; cell lysis; cell population analysis; cell-component sensing; continuous microfluidic channel; cytosolic component measurement; electrical pulse measurement; flow lysometry; single RBC; single-cell cytosolic analyzer; Biomedical measurements; Biomembranes; Cells (biology); Electric variables measurement; Fluorescence; Information analysis; Optical devices; Optical pumping; Plasma measurements; Pulse measurements;
Conference_Titel :
Micro Electro Mechanical Systems, 2009. MEMS 2009. IEEE 22nd International Conference on
Conference_Location :
Sorrento
Print_ISBN :
978-1-4244-2977-6
Electronic_ISBN :
1084-6999
DOI :
10.1109/MEMSYS.2009.4805320
