Title :
Micro-spectrophotometry applied to intracellular pH determination
Author :
Sun, H. ; Jacquey, F. ; Bernengo, J.C.
Author_Institution :
Inst. Nat. de la Sante et de la Rech. Medicale, Bron, France
fDate :
Oct. 29 1992-Nov. 1 1992
Abstract :
This paper presents intra-cellular pH measurements, performed through absorbance technique. Calibration data on rat myoballs have been obtained with a specially designed micro-spectro- photometer. Dye-protein interactions have been investigated, and dye titration curves inside living cells are presented. Absolute cytoplasmic pH determination are shown to be possible with an accuracy of 0.02 pH unit.
Keywords :
biochemistry; biological techniques; calibration; cellular biophysics; dyes; molecular biophysics; pH; proteins; spectrochemical analysis; spectrophotometry; absorbance technique; calibration; cytoplasmic pH determination; dye titration curves; dye-protein interactions; intracellular pH determination; microspectrophotometer; microspectrophotometry; rat myoballs; Accuracy; Biology; Electron tubes;
Conference_Titel :
Engineering in Medicine and Biology Society, 1992 14th Annual International Conference of the IEEE
Conference_Location :
Paris
Print_ISBN :
0-7803-0785-2
Electronic_ISBN :
0-7803-0816-6
DOI :
10.1109/IEMBS.1992.5760862
