Title :
A Fully Automated Micro-Solid Phase Extraction Chip for Genetic Sample Preparation System
Author :
Han, Song-I ; Lee, Hwanyoung ; Han, Ki-Ho
Author_Institution :
Sch. of Nano Eng., Inje Univ., Inje
Abstract :
This paper presents a fully automated micro-solid phase extraction (micro-SPE) chip for extracting genomic DNA from white blood cells. For realizing automated microfluidic control, high pressure dome-shaped microvalves are monolithically integrated. To increase the DNA extraction efficiency, silica beads were packed in the extraction microchannel involving two weir structures. Experimental results show that DNA extraction efficiency of the automated micro-SPE chip was 63%, averaged from five times successive measurement, in the first 8 mul elution solutions through automated SPE procedures. Furthermore, through the PCR-amplification of the beta-globulin gene, the extracted genomic DNA is verified that it is a good quality for downstream genetic assays.
Keywords :
DNA; bioMEMS; biological specimen preparation; blood; cellular biophysics; flow control; genomics; lab-on-a-chip; microchannel flow; microvalves; DNA extraction efficiency; PCR amplification; automated microfluidic control; automated microsolid phase extraction chip; beta globulin gene; downstream genetic assays; extraction microchannel; genetic sample preparation system; genomic DNA extraction; high pressure dome shaped microvalves; microSPE chip; monolithic integration; silica beads; white blood cell DNA; Automatic control; Bioinformatics; DNA; Genetics; Genomics; Microfluidics; Microvalves; Pressure control; Silicon compounds; White blood cells;
Conference_Titel :
Micro Electro Mechanical Systems, 2009. MEMS 2009. IEEE 22nd International Conference on
Conference_Location :
Sorrento
Print_ISBN :
978-1-4244-2977-6
Electronic_ISBN :
1084-6999
DOI :
10.1109/MEMSYS.2009.4805380
