A Fully Automated Micro-Solid Phase Extraction Chip for Genetic Sample Preparation System

Author

Han, Song-I ; Lee, Hwanyoung ; Han, Ki-Ho

Author_Institution

Sch. of Nano Eng., Inje Univ., Inje

fYear

2009

fDate

25-29 Jan. 2009

Firstpage

308

Lastpage

311

Abstract

This paper presents a fully automated micro-solid phase extraction (micro-SPE) chip for extracting genomic DNA from white blood cells. For realizing automated microfluidic control, high pressure dome-shaped microvalves are monolithically integrated. To increase the DNA extraction efficiency, silica beads were packed in the extraction microchannel involving two weir structures. Experimental results show that DNA extraction efficiency of the automated micro-SPE chip was 63%, averaged from five times successive measurement, in the first 8 mul elution solutions through automated SPE procedures. Furthermore, through the PCR-amplification of the beta-globulin gene, the extracted genomic DNA is verified that it is a good quality for downstream genetic assays.

Keywords

DNA; bioMEMS; biological specimen preparation; blood; cellular biophysics; flow control; genomics; lab-on-a-chip; microchannel flow; microvalves; DNA extraction efficiency; PCR amplification; automated microfluidic control; automated microsolid phase extraction chip; beta globulin gene; downstream genetic assays; extraction microchannel; genetic sample preparation system; genomic DNA extraction; high pressure dome shaped microvalves; microSPE chip; monolithic integration; silica beads; white blood cell DNA; Automatic control; Bioinformatics; DNA; Genetics; Genomics; Microfluidics; Microvalves; Pressure control; Silicon compounds; White blood cells;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems, 2009. MEMS 2009. IEEE 22nd International Conference on

Conference_Location

Sorrento

ISSN

1084-6999

Print_ISBN

978-1-4244-2977-6

Electronic_ISBN

1084-6999

Type

conf

DOI

10.1109/MEMSYS.2009.4805380

Filename

4805380