DocumentCode :
3043775
Title :
A Microfluidic Affinity Cocaine Sensor
Author :
Hilton, John P. ; Nguyen, ThaiHuu ; Pei, Renjun ; Stojanovic, Milan ; Lin, Qiao
Author_Institution :
Dept. of Mech. Eng., Columbia Univ., New York, NY
fYear :
2009
fDate :
25-29 Jan. 2009
Firstpage :
344
Lastpage :
347
Abstract :
We present a novel microfluidic sensor that is capable of detecting trace cocaine concentrations and is fully regenerable at modest temperatures. The sensor exploits affinity aptamers (synthetic DNA/RNA oligonucleotides), labeled with a fluorophore and immobilized onto polymer microbeads as a highly sensitive cocaine receptor medium. The device demonstrates the capability of detecting native cocaine concentrations as low as 100 pM, with an eight-orders-of-magnutide dynamic range. By way of enriching rarefied cocaine samples, this detection limit is further improved to 10 pM. Additionally, the device utilizes temperature-dependent reversibility of aptamer-analyte binding to regenerate the sensor surface at a relatively modest temperature (40degC). This feature allows repeated reuse of the sensor without loss in functionality.
Keywords :
chemical sensors; fluorescence; microfluidics; organic compounds; affinity aptamers; aptamer-analyte binding; cocaine receptor medium; fluorophore labeling; microfluidic affinity cocaine sensor; polymer microbeads; synthetic DNA oligonucleotides; synthetic RNA oligonucleotides; temperature dependent reversibility; Cobalt; DNA; Labeling; Mechanical sensors; Microfluidics; Optical sensors; RNA; Temperature distribution; Temperature sensors; Testing;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Micro Electro Mechanical Systems, 2009. MEMS 2009. IEEE 22nd International Conference on
Conference_Location :
Sorrento
ISSN :
1084-6999
Print_ISBN :
978-1-4244-2977-6
Electronic_ISBN :
1084-6999
Type :
conf
DOI :
10.1109/MEMSYS.2009.4805389
Filename :
4805389
Link To Document :
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