• DocumentCode
    3043866
  • Title

    Arraying Single Adherent Cells by Microplate Self-Assembly

  • Author

    Ishihara, Hirotaka ; Kuribayashi, Kaori ; Takeuchi, Shoji

  • Author_Institution
    Inst. of Ind. Sci., Univ. of Tokyo, Tokyo
  • fYear
    2009
  • fDate
    25-29 Jan. 2009
  • Firstpage
    367
  • Lastpage
    370
  • Abstract
    This paper describes a method for handling a single adherent cell using a self-assembly technique. We produce microplates using Parylene with both hydrophilic and hydrophobic sides. We culture the cells on the hydrophilic side, and utilize the hydrophobic side to self-assemble the plates onto the hydrophobic regions. Culturing a single cell on each Parylene microplate facilitates their handling since we can manipulate them as floating cells, and easily relocate them without the loss of cellular activity. In our experiment, we assemble 50 mum circle microplates with adherent cells in an array.
  • Keywords
    bioMEMS; cellular biophysics; hydrophilicity; hydrophobicity; polymers; self-assembly; Parylene microplate; cell array; cellular activity; floating cell; hydrophobic region; microplate self-assembly technique; single adherent cell; size 50 mum; Assembly; Glass; Gold; Lubricants; Plasma applications; Plasma properties; Self-assembly; Surface treatment; Tissue engineering; Toxic chemicals;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Micro Electro Mechanical Systems, 2009. MEMS 2009. IEEE 22nd International Conference on
  • Conference_Location
    Sorrento
  • ISSN
    1084-6999
  • Print_ISBN
    978-1-4244-2977-6
  • Electronic_ISBN
    1084-6999
  • Type

    conf

  • DOI
    10.1109/MEMSYS.2009.4805395
  • Filename
    4805395