DocumentCode
3043866
Title
Arraying Single Adherent Cells by Microplate Self-Assembly
Author
Ishihara, Hirotaka ; Kuribayashi, Kaori ; Takeuchi, Shoji
Author_Institution
Inst. of Ind. Sci., Univ. of Tokyo, Tokyo
fYear
2009
fDate
25-29 Jan. 2009
Firstpage
367
Lastpage
370
Abstract
This paper describes a method for handling a single adherent cell using a self-assembly technique. We produce microplates using Parylene with both hydrophilic and hydrophobic sides. We culture the cells on the hydrophilic side, and utilize the hydrophobic side to self-assemble the plates onto the hydrophobic regions. Culturing a single cell on each Parylene microplate facilitates their handling since we can manipulate them as floating cells, and easily relocate them without the loss of cellular activity. In our experiment, we assemble 50 mum circle microplates with adherent cells in an array.
Keywords
bioMEMS; cellular biophysics; hydrophilicity; hydrophobicity; polymers; self-assembly; Parylene microplate; cell array; cellular activity; floating cell; hydrophobic region; microplate self-assembly technique; single adherent cell; size 50 mum; Assembly; Glass; Gold; Lubricants; Plasma applications; Plasma properties; Self-assembly; Surface treatment; Tissue engineering; Toxic chemicals;
fLanguage
English
Publisher
ieee
Conference_Titel
Micro Electro Mechanical Systems, 2009. MEMS 2009. IEEE 22nd International Conference on
Conference_Location
Sorrento
ISSN
1084-6999
Print_ISBN
978-1-4244-2977-6
Electronic_ISBN
1084-6999
Type
conf
DOI
10.1109/MEMSYS.2009.4805395
Filename
4805395
Link To Document