Preliminary Production of Anti- Glufosinate Monoclonal Antibodies

Glutaraldehyde was used as cross linker to make glufosinate-ovalbumin. The identification and identified artificial antigen by UV abstraction and ³¹P NMR scanning methods, and then used this antigen immunize mice. The result showed that UV spectrogram of artificial antigen was changed when compared with OVA. 3 IP NMR spectrum of artificial antigen, the distance of artificial antigen and Glufosinate-ammonium were different. The conjugation ratio of artificial antigen was 36.7:1. After three weeks mice were immunized with artificial antigen and were examined that antibody was resided in the blood of mice (The titer of the antiserum is 1:320000). It could be concluded that Glufosinate-ammonium artificial antigen was synthesized and mice were immunized successfully, and this made it as a possible method to establish monoclonal antibodies.