Predicting Putative Target Genes of Activator Protein 1 Based on the dsDNA Microarray Data

Large scale identification of APltarget genes will be helpful for elucidating the whole biological function. But in natural enhancer regions, the sequences of API regulatory elements often deviate from the optimal recognition consensus sequence TGA(C/G) TCA. We therefore fabricated a double- stranded DNA (dsDNA) microarray with all possible single- nucleotide mutated (SNM) sequences of API consensus site and explored the binding affinity of these SNM sequences with one of API family protein, c-Jun. We selected the top four highest binding affinity SNM sequences and scanned their existences in the upstream 5 kb sequence of the known human genes in Homo sapiens. Those genes with the highest binding affinity sequence were deduced as putative target genes (PTGs) of API. Annotated with GO term relating with tumorigenesis we got some API PTGs likely correlated with carcinogenesis.