Telomerase Restrictors Might be A Novel Source for Screening Anti-HIV Agents

Author

Yu, Xin ; Wang, Jinghui ; De Giuli Morghen, Carlo ; Radaelli, Antonia ; Zanotto, Carlo ; Beggio, Paola

Author_Institution

Coll. of Pharmacy, Wuhan Univ., Wuhan

fYear

2007

fDate

6-8 July 2007

Firstpage

86

Lastpage

89

Abstract

Objectives: to screen anti-HIV agents from telomerase restrictors(TRs); Method: Selection of the maximum concentration of telomerase restrictor with minimum toxicity by means of MTT proliferation determination, and screening of anti-HIV agents by means of determining CEM-GFP fluorescence with FACS and through INNOTEST P24 HIV Antigen mAb EIISA. Results: Two telomerase restrictors were demonstrated to effectively restrict the proliferation of HIV. Conclusion: Telomerase restrictors (TR) might be the source for screening anti-HIV agents.

Keywords

biochemistry; cellular biophysics; diseases; enzymes; fluorescence; microorganisms; molecular biophysics; patient treatment; toxicology; CEM-GFP fluorescence; FACS; INNOTEST P24 HIV Antigen mAb EIISA; MTT proliferation determination; anti-HIV screening; telomerase restrictors; toxicity; Acquired immune deficiency syndrome; Biochemistry; Cancer; Educational institutions; Fluorescence; Human immunodeficiency virus; Immune system; Inhibitors; Instruments; Testing;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering, 2007. ICBBE 2007. The 1st International Conference on

Conference_Location

Wuhan

Print_ISBN

1-4244-1120-3

Type

conf

DOI

10.1109/ICBBE.2007.26

Filename

4272510