Title :
Telomerase Restrictors Might be A Novel Source for Screening Anti-HIV Agents
Author :
Yu, Xin ; Wang, Jinghui ; De Giuli Morghen, Carlo ; Radaelli, Antonia ; Zanotto, Carlo ; Beggio, Paola
Author_Institution :
Coll. of Pharmacy, Wuhan Univ., Wuhan
Abstract :
Objectives: to screen anti-HIV agents from telomerase restrictors(TRs); Method: Selection of the maximum concentration of telomerase restrictor with minimum toxicity by means of MTT proliferation determination, and screening of anti-HIV agents by means of determining CEM-GFP fluorescence with FACS and through INNOTEST P24 HIV Antigen mAb EIISA. Results: Two telomerase restrictors were demonstrated to effectively restrict the proliferation of HIV. Conclusion: Telomerase restrictors (TR) might be the source for screening anti-HIV agents.
Keywords :
biochemistry; cellular biophysics; diseases; enzymes; fluorescence; microorganisms; molecular biophysics; patient treatment; toxicology; CEM-GFP fluorescence; FACS; INNOTEST P24 HIV Antigen mAb EIISA; MTT proliferation determination; anti-HIV screening; telomerase restrictors; toxicity; Acquired immune deficiency syndrome; Biochemistry; Cancer; Educational institutions; Fluorescence; Human immunodeficiency virus; Immune system; Inhibitors; Instruments; Testing;
Conference_Titel :
Bioinformatics and Biomedical Engineering, 2007. ICBBE 2007. The 1st International Conference on
Conference_Location :
Wuhan
Print_ISBN :
1-4244-1120-3
DOI :
10.1109/ICBBE.2007.26
