Title :
Influence of Formaldehyde on the Expression of Rab26 Gene in HEK293 Cell in Vitro
Author :
Ying, Jiang ; Lifeng, Bao ; Kai, Wu ; Junlin, Yuan
Author_Institution :
Coll. of Life Sci., Huazhong Normal Univ., Wuhan
Abstract :
In this study HEK293 cell was treated with HCHO (ges 250 mu M) of different concentrations and Rab26 gene expression was examined by using RT-PCR method. The results showed that with the elevation of the concentrations of HCHO, the amount of DNA-protein crosslink (DPC) was significantly enhanced (P< 0.01). Meanwhile, accompanied with the elevation of DPC amount after HCHO treatment, the amount of replication and transcription of Rab26 was reduced as examined by PCR and RT-PCR method.
Keywords :
DNA; biochemistry; genetics; organic compounds; proteins; toxicology; DNA-protein crosslink; HEK293 cell; RT-PCR method; Rah26 gene expression; diseases; formaldehyde; replication; toxicities; transcription; Bioinformatics; Biological cells; DNA; Fluorescence; Genetic mutations; Genomics; In vitro; Polymers; Proteins; RNA;
Conference_Titel :
Bioinformatics and Biomedical Engineering, 2007. ICBBE 2007. The 1st International Conference on
Conference_Location :
Wuhan
Print_ISBN :
1-4244-1120-3
DOI :
10.1109/ICBBE.2007.57
