Title :
Primary study on thermophiles during pile-fermentation of Pu-er tea
Author :
Chen, Chaoyin ; Zhang, Wenming ; Chanyong Lu ; Han, Benyong ; Xiong, Xianfeng ; Ge, Feng ; Zhao, Shenglan ; Chen, D.
Author_Institution :
Fac. of Life Sci. & Technol, Kunming Univ. of Sci. & Technol., Kunming, China
Abstract :
Pu-er tea pile-fermentation is a high temperature process. Thermophiles during the pile-fermentation of Pu-er tea have not been reported up to now. This paper was isolated 13 thermophiles during the pile-fermentation of Pu-er. Then each strain was inoculated into the piles of Pu-er tea and simulated thermophilic pile-fermentation, with testing tea polyphenols, theaflavin, thearubigins and theabrownines. The content of theabrowin of the group added the 13 strains greatly increased to 22.73%. Six more favorable and important strains were identified by 16S rDNA sequencing. The results indicated that three belong to Bacillus, the others to Thermoactinomyces, Streptomyces, Ureibacillus, respectively.
Keywords :
beverages; fermentation; microorganisms; 16S rDNA sequencing; Bacillus; Pu-er tea; Streptomyces; Thermoactinomyces; Ureibacillus; high-temperature process; polyphenols; simulated thermophilic pile-fermentation; theabrowin content; theabrownines; theaflavin; thearubigins; thermophiles; Microorganisms; Pigments; Strain; Surface morphology; Surface treatment; Temperature measurement; Turning; Pile-Fermentation; Pu-er tea; Thermophiles;
Conference_Titel :
IT in Medicine and Education (ITME), 2011 International Symposium on
Conference_Location :
Cuangzhou
Print_ISBN :
978-1-61284-701-6
DOI :
10.1109/ITiME.2011.6132094
