Title :
Rapid real-time PCR-based nucleotide sequence measurement method using 1480nm infrared laser heating
Author :
Terazono, H. ; Hattori, A. ; Takei, H. ; Takeda, K. ; Yasuda, K.
Author_Institution :
Tokyo Med. & Dental Univ., Tokyo
Abstract :
The polymerase chain reaction (PCR) is the key technology in genome-based biological analysis, and has revolutionized many life science applications like medical diagnostics, food hygiene, and anti bio terrorism. For the practical applications of PCR, one of the most important key factors is the time of data acquisition. Thus we have developed novel rapid real-time PCR-based nucleotide sequence measurement method, which have a possibility applied to rapid gene analysis such as sanitary survey or gene diagnosis.
Keywords :
DNA; biomedical measurement; laser applications in medicine; thermal diffusion; annealing; data acquisition time; extension temperature; gene diagnosis; genome-based biological analysis; infrared laser heating; laser irradiation; nucleotide sequence measurement method; polymerase chain reaction; thermal diffusion; Bioinformatics; Biology; Food technology; Genomics; Infrared heating; Laser theory; Medical diagnosis; Nuclear measurements; Polymers; Terrorism;
Conference_Titel :
Microprocesses and Nanotechnology, 2007 Digest of papers
Conference_Location :
Kyoto
Print_ISBN :
978-4-9902472-4-9
DOI :
10.1109/IMNC.2007.4456236