DocumentCode :
305313
Title :
Real time excitation-emission spectra for in vivo disease diagnosis
Author :
Itzkan, Irving
Author_Institution :
MIT, MA, USA
Volume :
1
fYear :
1996
fDate :
18-21 Nov. 1996
Firstpage :
330
Abstract :
It has been demonstrated that fluorescence spectroscopy is able to distinguish between diseased and healthy tissue and now is being explored as a potential tool for the diagnosis of disease. This often requires complete characterization of the absorption and fluorescence properties of normal and abnormal tissues over the near UV and visible regions. Such information is often collected and displayed in the form of an excitation emission matrix (EEM). We have designed, fabricated, and tested a compact, transportable EEM system capable of collecting multi-excitation spectra in approximately 600 milliseconds in a reliable manner. This is an increase in data collection speed of at least an order of magnitude over some other previous systems and is an essential advantage when measuring spectroscopic signals in vivo. Spectra can be collected from the same tissue site, without artifacts due to probe displacement caused by heartbeats, breathing and other body motions, and without undue strain on the patience of the physician and patient. A white light source provides simultaneous measurement of diffuse reflectance from the same tissue site on each cycle, giving important additional diagnostic information. Our system uses a 337 nm nitrogen laser to pump a sequence of ten dye cuvettes located on a rotating wheel. The pump is also used as an excitation source, providing a total of eleven excitation wavelengths approximately uniformly spaced in the range 337 to 505 nm. By changing the dyes used, the excitation wavelengths can be varied as required.
Keywords :
biomedical measurement; data acquisition; fluorescence spectroscopy; laser applications in medicine; patient diagnosis; real-time systems; ultraviolet spectroscopy; visible spectroscopy; 337 nm N/sub 2/ laser; 337 to 505 nm; 600 ms; N/sub 2/; artifacts; body motions; breathing; compact transportable EEM system; data collection speed; diffuse reflectance; diseased tissue; dye cuvettes; excitation emission matrix; excitation wavelengths; fluorescence spectroscopy; healthy tissue; heartbeats; in vivo disease diagnosis; multi-excitation spectra; probe displacement; real time excitation-emission spectra; rotating wheel; spectroscopic signals; tissue site; white light source; Capacitive sensors; Diseases; Electromagnetic wave absorption; Fluorescence; In vivo; Laser excitation; Probes; Spectroscopy; System testing; Velocity measurement;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Lasers and Electro-Optics Society Annual Meeting, 1996. LEOS 96., IEEE
Conference_Location :
Boston, MA, USA
Print_ISBN :
0-7803-3160-5
Type :
conf
DOI :
10.1109/LEOS.1996.565267
Filename :
565267
Link To Document :
بازگشت