Ceramide channel formed in open well thermal plastic chips

In this paper, we report a nanopore study platform in a microfluidic chip to gain easier control over temperature and flow conditions. Micro channels are milled with Computer Numerical Control machine on two polycarbonate wafers and then sealed into a two-chamber system by thermal bonding with a PVDC as partition. A round hole of 80-100 ¿m in diameter was burnt by hot needle at the cross section of two channels and will hold the PPM during the experiment. Two nanoports (Upchurch, WA) holding Ag/AgCl electrodes are fixed at ends of micro channels by wax and easy to remove and re-apply.