Title :
The damage of DNA induced by UV nanosecond laser excitation at 193 nm
Author :
Vtyurina, N.N. ; Grokhovski, S.L. ; Filimonov, Ilya V. ; Medvedkov, O.I. ; Nechipurenko, D.Y. ; Vasiliev, S.A. ; Nechipurenko, Y.D.
Author_Institution :
Fac. of Phys., Moscow State Univ., Moscow, Russia
Abstract :
Solutions containing dsDNA fragments were irradiated with UV laser pulses. The produced damage was investigated using polyacrylamid gel electrophoresis. The intensity of cleavage in particular phosphodiester bond after hot alkali treatment of irradiated samples was shown to depend on base pair sequence. The damage of DNA fragments has occurred predominantly in the sites containing guanine clusters.
Keywords :
DNA; biological effects of laser radiation; biological effects of ultraviolet radiation; biomolecular effects of radiation; bonds (chemical); electrophoresis; molecular configurations; DNA damage; UV nanosecond laser excitation; base pair sequence; dsDNA fragments; guanine clusters; hot alkali treatment; phosphodiester bond cleavage; polyacrylamid gel electrophoresis; wavelength 193 nm; DNA; Lasers;
Conference_Titel :
Advanced Optoelectronics and Lasers (CAOL), 2010 International Conference on
Conference_Location :
Sevastopol
Print_ISBN :
978-1-4244-7043-3
DOI :
10.1109/CAOL.2010.5634194
