High resolution contrast ultrasound and NADH fluorescence imaging of myocardial perfusion in excised rat hearts

Simultaneous imaging of myocardial flow and hypoxia could be vital for identifying acute ischemic mechanisms that may trigger an arrhythmia. We have studied the distribution of flow and hypoxia in excised locally ischemic rat hearts using simultaneous contrast ultrasound imaging and beta-nicotinamide adenine dinucleotide (NADH) fluorescence imaging. Local ischemia was induced by controlling flow within a major coronary artery. Intra-myocardial flow was imaged using contrast high-resolution ultrasound (linear probe; 13−6 MHz). An ultrasound contrast agent (UCA) was used to highlight the ischemic border. We observed distinct borders between two perfusion beds. UCA images showed high contrast borders of flow. The progression of UCA through the tissue was clearly visible. Intramyocardial regions of flow overlap could be identified by superimposing images of UCA from two perfusion zones. Borders between hypoxic and normoxic tissue were clearly revealed by increased NADH fluorescence. Hypoxic borders were oriented along borders of flow. In summary, simultaneous ultrasound and NADH imaging of excised hearts from small animals provide high fidelity images for characterizing the distribution of flow and hypoxic tissue during acute localized ischemia.