Title :
Dependence of the photobleaching of fluorescent proteins on the repetition rate of femtosecond light pulses
Author :
Toda, Keisuke ; Takahashi, Hiroki ; Suda, Akira
Author_Institution :
Dept. of Phys., Tokyo Univ. of Sci., Noda, Japan
fDate :
June 30 2013-July 4 2013
Abstract :
We investigate the photobleaching of fluorescent proteins as functions of pulse interval, chirp, and pulse energy of femtosecond excitation lights and find out that the photobleaching occurs via one-photon excited-state absorption of the triplet state.
Keywords :
fluorescence; high-speed optical techniques; molecular biophysics; optical saturable absorption; proteins; femtosecond excitation light; femtosecond light pulse; fluorescent proteins; one-photon excited-state absorption; photobleaching; pulse chirp; pulse energy; pulse interval; repetition rate; triplet state; Absorption; Chirp; Delays; Fluorescence; Microscopy; Photobleaching; Proteins;
Conference_Titel :
Lasers and Electro-Optics Pacific Rim (CLEO-PR), 2013 Conference on
Conference_Location :
Kyoto
DOI :
10.1109/CLEOPR.2013.6600622
