Title :
Microbubble-mediated oxygen delivery to hypoxic tissues as a new therapeutic device
Author :
Bisazza, Agnese ; Giustetto, Pierangela ; Rolfo, Alessandro ; Caniggia, Isabella ; Balbis, Sonia ; Guiot, Caterina ; Cavalli, Roberta
Author_Institution :
Dept Drug Science and Technology, University of Torino, Italy
Abstract :
Chitosan-coated oxygen microbubbles of average diameter 2.5 μm, narrow size distribution and spherical shape were prepared. A core-shell structure was evidenced by fluorescence microscopy using fluorescent microbubbles. Such microbubbles can be a therapeutic device for vehiculating oxygen to hypoxic tissues, provided they show proper permeability and diffusivity properties and are non-toxic. Our study proves that oxygen is efficiently delivered both in ‘in vitro’ and ‘in vivo’ preparations, and can be conveniently metabolized reversing the cellular hypoxic response. Moreover, toxic effects were investigated in human blood and in cultured cells and no evidence for them was found.
Keywords :
Biomedical monitoring; Blood; Cells (biology); Drugs; Fluorescence; Gynaecology; Humans; Neuroscience; Pharmaceutical technology; Transmission electron microscopy; delivery; microbubble; oxygen; release; Animals; Anoxia; Cells, Cultured; Chitosan; Drug Carriers; Eggs; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Microbubbles; Oxygen;
Conference_Titel :
Engineering in Medicine and Biology Society, 2008. EMBS 2008. 30th Annual International Conference of the IEEE
Conference_Location :
Vancouver, BC
Print_ISBN :
978-1-4244-1814-5
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2008.4649599
