Automation of fluorescent dot counting in cell nuclei

Author

Netten, Hans ; Young, Ian T. ; Prins, Michele ; Van Vliet, Lucas J. ; Tanke, Hans ; Vrolijk, Hans ; Sloos, Willem

Author_Institution

Fac. of Appl. Phys., Delft Univ. of Technol., Netherlands

Volume

1

fYear

1994

fDate

9-13 Oct 1994

Firstpage

84

Abstract

We have developed a completely automated fluorescence microscope system that can examine 500 cells in approximately 20 minutes to determine the number of labeled chromosomes (seen as dots) in each cell nucleus. This system works with two fluorescent dyes-one for the DNA hybridization dots (e.g. FITC) and one for the cell nucleus (e.g. DAPI). After the stage has moved to a new field the image is automatically focused, acquired by a Photometrics KAF 1400 camera, and then analyzed on a Macintosh Quadra 840AV computer. After the required number of cells has been analyzed, the user may interact to correct the computer by working with a gallery of the cell images. The machine accuracies are equal to panels of human experts (manual) and limited by the overlapping of dots in the 3D cell as seen through the 2D projection

Keywords

computer vision; DNA hybridization dots; Macintosh Quadra 840AV computer; Photometrics KAF 1400 camera; automatic fluorescent dot counting; cell images; cell nuclei; fluorescence microscope system; labeled chromosomes; Automation; Biological cells; Cameras; Cells (biology); DNA; Fluorescence; Focusing; Image analysis; Microscopy; Photometry;

fLanguage

English

Publisher

ieee

Conference_Titel

Pattern Recognition, 1994. Vol. 1 - Conference A: Computer Vision & Image Processing., Proceedings of the 12th IAPR International Conference on

Conference_Location

Jerusalem

Print_ISBN

0-8186-6265-4

Type

conf

DOI

10.1109/ICPR.1994.576231

Filename

576231