Title :
Applications of optical resonance to biological imaging and label-free protein microarrays
Author :
Ünlü, M. Selim ; Özkumur, I.E. ; Needham, J. ; Bergstein, D.A. ; Goldberg, B.B. ; Yalcin, A. ; Spuhler, P. ; Irani, R. ; DeLisi, C.
Author_Institution :
Departments of Electrical and Computer Engineering, Physics and Biomedical Engineering at Boston University, MA 02215, USA
Abstract :
We present biological imaging and sensing methods based on optical resonance and interference. In fluorescence microscopy, our nanoscale imaging capability sheds light onto conformational changes of DNA, DNA-protein complexes and polymer coatings on a solid surface. Interference measurements on a layered substrate yield a label-free sensing platform for protein binding in a high-throughput micro-array format.
Keywords :
Biomedical optical imaging; Fluorescence; Interference; Microscopy; Nanobioscience; Optical imaging; Optical polymers; Optical sensors; Proteins; Resonance; Animals; Biosensing Techniques; Electrochemistry; Fluorescence; Fluorescent Dyes; Humans; Microscopy, Fluorescence; Nucleic Acid Hybridization; Optics and Photonics; Protein Array Analysis; Proteins;
Conference_Titel :
Engineering in Medicine and Biology Society, 2008. EMBS 2008. 30th Annual International Conference of the IEEE
Conference_Location :
Vancouver, BC
Print_ISBN :
978-1-4244-1814-5
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2008.4649744
