Applications of optical resonance to biological imaging and label-free protein microarrays

Author

Ünlü, M. Selim ; Özkumur, I.E. ; Needham, J. ; Bergstein, D.A. ; Goldberg, B.B. ; Yalcin, A. ; Spuhler, P. ; Irani, R. ; DeLisi, C.

Author_Institution

Departments of Electrical and Computer Engineering, Physics and Biomedical Engineering at Boston University, MA 02215, USA

fYear

2008

fDate

20-25 Aug. 2008

Firstpage

2643

Lastpage

2643

Abstract

We present biological imaging and sensing methods based on optical resonance and interference. In fluorescence microscopy, our nanoscale imaging capability sheds light onto conformational changes of DNA, DNA-protein complexes and polymer coatings on a solid surface. Interference measurements on a layered substrate yield a label-free sensing platform for protein binding in a high-throughput micro-array format.

Keywords

Biomedical optical imaging; Fluorescence; Interference; Microscopy; Nanobioscience; Optical imaging; Optical polymers; Optical sensors; Proteins; Resonance; Animals; Biosensing Techniques; Electrochemistry; Fluorescence; Fluorescent Dyes; Humans; Microscopy, Fluorescence; Nucleic Acid Hybridization; Optics and Photonics; Protein Array Analysis; Proteins;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2008. EMBS 2008. 30th Annual International Conference of the IEEE

Conference_Location

Vancouver, BC

ISSN

1557-170X

Print_ISBN

978-1-4244-1814-5

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/IEMBS.2008.4649744

Filename

4649744