Title :
Volumetric image distortion due to refractive index mismatch in 3D confocal scanning laser microscopy
Author :
Doukoglou, Tilemachos D. ; Hunter, Ian W.
Author_Institution :
Dept. of Biomed. Eng., McGill Univ., Montreal, Que., Canada
Abstract :
In the application of 3D-volume reconstruction from 2D image slices acquired by a confocal scanning laser microscope errors arise due to refractive index mismatch. This paper presents a brief theoretical analysis of this error that can lead to miscalculating a specimen´s 3D geometry. A comparison between the error analysis and experimental measurements is also provided
Keywords :
biological techniques; biology computing; error analysis; image reconstruction; measurement errors; medical image processing; optical images; optical microscopy; physics computing; refractive index; 2D image slices; 3D confocal scanning laser microscopy; 3D geometry; 3D-volume reconstruction; confocal scanning laser microscope; error analysis; refractive index mismatch; volumetric image distortion;
Conference_Titel :
Engineering in Medicine and Biology Society, 1995., IEEE 17th Annual Conference
Conference_Location :
Montreal, Que.
Print_ISBN :
0-7803-2475-7
DOI :
10.1109/IEMBS.1995.575222
