Title :
Design of electrical stimulation bioreactors for cardiac tissue engineering
Author :
Tandon, N. ; Marsano, A. ; Cannizzaro, C. ; Voldman, J. ; Vunjak-Novakovic, G.
Author_Institution :
Columbia University, Dept. of Biomedical Engineering, 1210 Amsterdam Ave, MC 8904, New York 10027, USA
Abstract :
Electrical stimulation has been shown to improve functional assembly of cardiomyocytes in vitro for cardiac tissue engineering. Carbon electrodes were found in past studies to have the best current injection characteristics. The goal of this study was to develop rational experimental design principles for the electrodes and stimulation regime, in particular electrode configuration, electrode ageing, and stimulation amplitude. Carbon rod electrodes were compared via electrochemical impedance spectroscopy (EIS) and we identified a safety range of 0 to 8 V/cm by comparing excitation thresholds and maximum capture rates for neonatal rat cardiomyocytes cultured with electrical stimulation. We conclude with recommendations for studies involving carbon electrodes for cardiac tissue engineering.
Keywords :
Aging; Assembly; Bioreactors; Cardiac tissue; Cardiology; Design engineering; Design for experiments; Electrical stimulation; Electrodes; In vitro; Animals; Animals, Newborn; Bioreactors; Carbon; Electric Stimulation; Electricity; Electrochemistry; Electrodes; Equipment Design; Models, Chemical; Myocardium; Myocytes, Cardiac; Rats; Temperature; Tissue Engineering;
Conference_Titel :
Engineering in Medicine and Biology Society, 2008. EMBS 2008. 30th Annual International Conference of the IEEE
Conference_Location :
Vancouver, BC
Print_ISBN :
978-1-4244-1814-5
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2008.4649983