Title :
Resonance Scattering Spectral Detection of Ultratrace CEA Using Immunonanogold-VC-HAuCl4 Catalytic Reaction
Author :
Liang, Aihui ; Jiang, Caina ; Zhang, Yi ; Jiang, Zhiliang
Author_Institution :
Key Lab. of Ecology of Rare & Endangered Species & Environ. Protection, Guangxi Normal Univ., Guilin, China
Abstract :
A 10 nm nanogold particle was used to label monoclone CEA antibody to prepare an immunonanogold resonance scattering probe (AuCEAAb) for detection of CEA. In the pH 6.8 Na2HPO4-NaH2PO4 buffer solution and in the presence of PEG-6000, the immunoreaction between CEA and AuCEAAb took place to form the immunonanogold complex that can be removed by centrifugation technique. The supernatant containing AuCEAAb exhibited catalytic effect on the gold particle reaction of HAuCl4-VC in the presence of cetyltrimethylammonium bromide (CTMAB). The gold particle exhibited a resonance scattering peak at 600 nm. Using the resonance scattering spectral technology as the detection, a new immunonanogold catalytic resonance scattering method was established to detect CEA. Under the chosen conditions, the resonance scattering intensity at 600 nm (I600 nm) decreased with the CEA concentration increasing. The decreased intensity ΔI600 nm was linear to CEA concentration (CCEA) in the range of 0.064-3.2 ng·mL-1, with the regression equation of ΔI600 nm = 32.5 CCEA + 2.56, correlation coefficient of 0.9945, and detection limit of 0.01 ng·mL-1. This method was applied to the analysis of CEA in serum samples, with satisfactory results.
Keywords :
bio-optics; biochemistry; biomedical materials; biosensors; catalysis; gold; light scattering; molecular biophysics; nanobiotechnology; nanoparticles; proteins; regression analysis; catalytic reaction; centrifugation technique; correlation coefficient; immunonanogold catalytic resonance scattering method; immunonanogold resonance scattering probe; immunoreaction; monoclone CEA antibody; nanogold particle; regression equation; resonance scattering spectral detection; serum; supernatant; ultratrace CEA; Gold; Immune system; Instruments; Nanobioscience; Nanoparticles; Neoplasms; Particle scattering; Proteins; Resonance; Virtual colonoscopy;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5514701