Public software: TomoJ, eTomo and tomtoolbox for stem and EFTEM tomography of biological samples

Since 3D Electron Tomography Reconstruction is currently under development, several software are appearing in this field. Hereby, we present a study of recent applications used in electron tomography. We have highlighted EFTEM and TomoJ Plug-in under ImageJ, Tomography Toolbox (Tom toolbox) under MATLAB and eTomo Under IMOD. We have also applied preprocessing and reconstruction steps for real data (biological samples) by using one of the programs aforementioned, therefore according to the acquired results, we have compared these programs in terms of Electron Tomography Reconstruction. The goal of this comparison is to develop hybrid software that takes the advantages of all previous software. Furthermore, we have summarized Advantages and disadvantages of the introduced software.